https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31426 CBE1 expression in normal as well as impaired spermatogenesis on mRNA (quantitative reverse-transcription polymerase chain reaction and in situ hybridization) and protein level (immunohistochemistry, Western blot analysis). Result(s): In normal spermatogenesis, CBE1 mRNA was expressed in late pachytene spermatocytes, and the protein was localized within the flagellum of elongating spermatids from stage V up to the spermiation in stage II. Immunoelectron microscopy showed CBE1 clearly associated with microtubules at the manchette, the head-tail coupling apparatus, and the flagellum, but the protein was absent in spermatozoa. Compared with normal spermatogenesis, CBE1 mRNA was statistically significantly reduced in samples with a maturation arrest at the level of round spermatids and primary spermatocytes, and was absent in samples showing Sertoli cell-only syndrome. CBE1 protein was completely missing in SDA samples showing few elongating spermatids. Conclusion(s): Our data strongly suggest an influence of CBE1 in ciliogenesis in spermatids due to the localization at the microtubules of the elongating spermatids, indicating a role in the intramanchette and/or intraflagellar transport mechanism. The absence of CBE1 in spermatozoa suggests that CBE1 is important for the spermatid development but not for the maintenance of mature spermatozoa as a component of the flagellum.]]> Wed 11 Jul 2018 17:04:16 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18882 Wed 11 Apr 2018 15:08:03 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15139 Wed 11 Apr 2018 14:39:08 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41662 Wed 10 Aug 2022 11:10:24 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37820 Wed 09 Feb 2022 15:58:38 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51128 6,000 proteins, encompassing the selective loss and gain of several hundred proteins. Further, we demonstrate epididymal-driven activation of RHOA-mediated signaling pathways is an important component of sperm maturation. These data contribute molecular insights into the complexity of proteomic changes associated with epididymal sperm maturation.]]> Tue 22 Aug 2023 15:52:22 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33498 Tue 06 Nov 2018 11:19:37 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44824 Thu 27 Oct 2022 12:44:28 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52637 Thu 19 Oct 2023 15:11:13 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54274 Thu 15 Feb 2024 14:39:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37797 Mon 26 Apr 2021 12:23:37 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54975 Mon 25 Mar 2024 15:21:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36447 Mon 04 May 2020 16:14:04 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45427 Fri 28 Oct 2022 11:48:22 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40160 Fri 22 Jul 2022 13:28:02 AEST ]]>